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KMID : 0381120170390080903
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Genes and Genomics
2017 Volume.39 No. 8 p.903 ~ p.911
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A conserved repetitive DNA element located in the centromeres of chromosomes in Medicago genus
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Yu Feng
Dou Quanwen
Liu Ruijuan
Wang Haiqing
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Abstract
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In most eukaryotic species, centromeres harbor large arrays of tandem repeated satellite DNA sequences. In this study, we report on the genomic distribution of a centromere satellite repeat “MtR3” in Medicago genus and three distantly related genera. Fluorescence in situ hybridization (FISH) results showed MtR3 repeats were detected in the centromere regions in M. truncatula, M. minima, M. edgeworthii, M. ruthenica, M. caerulea, M. sativa, and M. falcata (4×), but no signals were discovered in M. lupulina, M. polymorpha, and M. falcata (2×), Melilotus officinalis, Crotalaria medicaginea, and Trifolium repens. However, sequence analysis showed this MtR3 DNA had genomic distribution in all species and was highly conserved across the entire Medicago genus and three other genera. The conservation and widespread presence suggested MtR3 repeats may play important roles in centromeric function.
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KEYWORD
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Medicago, Centromere, Fluorescence in situ hybridization, Satellite repeat
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